عالية عيسى بشبوش شمس الدين

Background: The emergence of antibiotic resistance among pathogenic bacteria has become a serious problem worldwide. Integrons are useful marker of antibiotic resistance dissemination among Enterobacteriaceae. The purpose of current study was to compare the existence of the intI1andintI2 gene and its associations with the resistant to antibiotic of Escherichia coli isolated from patients with suspected with UTIs. Methods: For the period of the study about of 1300 urine specimens were collected from patients suspected with UTIs, Kirby-Bauer disk diffusion method 28 antimicrobial agents used to determine the antibiotic susceptibility of E coli isolates. The isolates had be subjected to polymerase chain reactions (PCR) for detection of class 1 and 2 integron and antibiotic resistance genes. Results: From 1300 urine specimens E. coli isolates had represented in 111(41.3%). The antimicrobial sensitivity profile of 111 E. coli isolates were studied 104(93.3%) were multidrug resistant (MDR) and five (4.4%) isolates were susceptible to all antibiotics. The PCR data of integrons genes revealed that 89 (85.6%) of MDR E. coli isolates had integrons genes, intI1 was detected in 82.7% of isolates, while intI2 was found only in 6.7% of them. Detect the bouth of intI1 and intI2 had detected in 3.8% of the isolates. The qacEΔ1 gene were found in 65.1%, sul1 gene were detected in 8.1% ,and variable region in a different size products were detected in 89.5% of class I integron, at the same time as for variable regions of class II integron were detected in 57.1%. Conclusions: The study is show that the high frequency of class I integron in Uropathogenic Escherichia coli (UPEC) isolates conferring resistance to some classes of antibiotic.

Background: The emergence of antibiotic resistance among pathogenic bacteria has become a serious problem worldwide. Integrons are useful marker of antibiotic resistance dissemination among Enterobacteriaceae. The purpose of current study was to compare the existence of the intI1andintI2 gene and its associations with the resistant to antibiotic of Escherichia coli isolated from patients with suspected with UTIs. Methods: For the period of the study about of 1300 urine specimens were collected from patients suspected with UTIs, Kirby-Bauer disk diffusion method 28 antimicrobial agents used to determine the antibiotic susceptibility of E coli isolates. The isolates had be subjected to polymerase chain reactions (PCR) for detection of class 1 and 2 integron and antibiotic resistance genes. Results: From 1300 urine specimens E. coli isolates had represented in 111(41.3%). The antimicrobial sensitivity profile of 111 E. coli isolates were studied 104(93.3%) were multidrug resistant (MDR) and five (4.4%) isolates were susceptible to all antibiotics. The PCR data of integrons genes revealed that 89 (85.6%) of MDR E. coli isolates had integrons genes, intI1 was detected in 82.7% of isolates, while intI2 was found only in 6.7% of them. Detect the bouth of intI1 and intI2 had detected in 3.8% of the isolates. The qacEΔ1 gene were found in 65.1%, sul1 gene were detected in 8.1% ,and variable region in a different size products were detected in 89.5% of class I integron, at the same time as for variable regions of class II integron were detected in 57.1%. Conclusions: The study is show that the high frequency of class I integron in Uropathogenic Escherichia coli (UPEC) isolates conferring resistance to some classes of antibiotic.

ABSTRACT— The Epstein-Barr virus (EBV) is responsible for the majority of human malignancies. Chemotherapy patients have low white blood cell (WBC) counts, which increases the risk of infection and is Iraq's top public health issue. WBC can destroy tumors and assist in infection control, but they also increase tumor development, metastasis, and treatment resistance. The body is more susceptible to infection when the lymphocytes count is low. The goal of our research was to show if there was a link between low lymphocyte counts and EBV infection in cancer patients. The study included patients (cross-sectional study). The Middle Euphrates Center for Cancer split the participants into two groups: 90 cancer patients with Lymphocytopenia and 90 (no Lymphocytopenia) 180 blood samples were taken, a blood sample from a cancer patient undergoing chemo (period between February, 2021 and August, 2021). The patients' ages varied from 10 to 70. Subjects' samples were divided into In the CBC test, entire blood samples were tested for EBV Ags, IgM, and IgG, CBC test, amount of lymphocytes is 1000/mcL (1 109/L). Using commercial ELISA kits, patients' blood samples were tested for EBV Ag, IgM, and IgG antibodies. This research examined three Lymphocytopenia symptoms (mild, moderate, and severe). Mild patients made up 35.6%, moderate 30%, and severe 34.4%. The three degrees of Lymphocytopenia were categorized by age groups; with category (51-70) having the most severe cases 43.3%. The 31-50 age groups came in second with 33.3 %. These tests looked for EBV Ag, IgM antibody, and IgG antibody in all three Lymphocytopenia grades. The original data showed 90 people with 29 IgM-positive cases (38%), 43IgG-positive cases 52%, and EBV Ag positive cases 18 it found mild results in 32 cases, including 9 IgM and 11 IgG positives. There were also 7 EBV Ag positive patients. The moderate group included 9 IgM positive, 11 IgG positive, and 7 EBV positive patients. In terms of severity, 10 individuals had IgM, 18 had IgG, and 3 had Ags. According to the results of the current investigation, cancer patients with Lymphocytopenia were more likely to have viral infections than cancer patients without Lymphocytopenia.

تم خلال فترة الدراسة جمع وفحص 226عينة ساءل نخاع شوكي من الاطفال المرضى الذين دخلو مستشفى الولادة والاطفال خلال الفترة من 11-2001--- -1-2004 حيث كانت نسبة الاصابة بالتهاب السحايا 11,01%من المجموع الكلي للاطفال لبكتريا التي تم عزلها كانت كلالتي E.coli,Klebsiella pneumonia,Enterobacter ssp,Acinetobactercalcoaceticus.Heamophylus influenza.Streptococcus Pneumoniae .كما اظهرت الدراسة ان.Enterobacter spp Staphylococcus aureus شكلا نسب 64%من عينات سائل النخاع الشوكي وان نسبة الاصابة بين الذكور كانت 52% وبالاناث 48%