Gender-related response to different wavelength low-level laser therapy in orthodontic mandibular canine movement: a split mouth study
AuthorsAlmustafa Alhumadi
JournalEndodontics today
Date2026-01-06
AbstractINTRODUCTION. Low-level laser therapy (LLLT), known as photobiomodulation (PBM), has recently attracted
the attention of clinicians to speed up orthodontic tooth movement. Nevertheless, the sex-related effects of
LLLT have not been investigated yet, although the role of sex hormones on bone remodeling has already been
reported. The objective of this study was to determine if LLLT preferentially accelerates the rate of mandibular
canine retraction in men or women.
MATERIALS AND METHODS. Twenty systemically healthy patients (10 male, 10 females; age range from 18 to
25 years) who were undergoing fixed orthodontic treatment were included in a split-mouth randomized con-
trol trial. Subjects were stratified into two groups: Group 1 (n
=
10) was treated with 650 nm LLLT on the ex-
perimental side, while group 2 (n
=
10) was treated with 976 nm LLLT. Opposite sides of the animals were used
as a sham-irradiated control. PBM parameters were compatible (8 J/cm2 energy density, 0.26 W/cm2 power
density) and administered on d 0, 3, 7, 14, then bi-weekly thereafter. Retraction of canine was done with 150 g
force produced by elastics. Amount of linear tooth movement was evaluated at 1 month and space closure
duration was monitored until complete closure.
RESULTS. The amount of the canine movement was significantly higher on LLLT treated sides compared to
control ones after 1 month (p
<
0.001, mean difference: 0.92
±
0.46 mm; 1.44
±
0.29 mm vs control sides, re-
spectively). Better acceleration was obtained at 976 nm (1.12
±
0.44 mm gain) vs. 650 nm (0.71
±
0.39 mm;
p
<
0.001). LLLT sides closed spaces in 3.3 months, whereas controls took 6.2 months for complete closure.
Sex stratification found that the accelerations were consistently higher in waves for both males and females
indicating no sex-by-wavelength interactions.
CONCLUSIONS. LLLT efficiently speeds up the retraction of the mandibular canine, and the 976 nm wave-
length works best. Sex does not affect the effectiveness of LLLT, which supports the consistent use of similar
procedures across different groups of patients.
Keywords: photobiomodulation, orthodontic tooth movement, canine retraction, sex differences, laser
therapy, near-infrared
Determination of Minimum Inhibitory Concentration and Minimum Bactericidal Concentration of Zinc Oxide Nanoparticles from Internal Cavity of Dental Implant and Natural Teeth (in vitro study)
AuthorsDhyaa M. J. Hirz AL-Deen 1, , Marwah Safaa Ali 2, Almustafa Alhumadi 3*
JournalJournal of nanostructures
Date2025-10-01
AbstractNanotechnology characterizes a new knowledge that potentials to deliver
a wide range of uses and improved technologies for biological and
biomedical applications. Nanotechnology permits synthesis of materials
that have structure is less than 100 nanometers. The present work revealed
the determination minimum inhibitory concentration and maximum
bactericidal concentration of zinc oxide nanoparticles for aerobic and
anaerobic bacteria isolated from internal cavity of the implant and sulcus
of the natural teeth. Bacteria were isolated from internal hole of the implant
after 90 days from surgical placement for 16 dental implant and sulcus of
natural teeth for 6 female age range 30-44years. Different concentrations
of ZnO NPs were prepared in solvent (water 3:1 ethanol) and mixed with
brain heart infusion agar all the experiments were conducted in vitro. Agar
dilution method was used to study the minimum inhibitory concentration
and minimum bactericidal concentration and MBC for tested bacteria. The
MIC for aerobic and anaerobic bacteria isolated from internal cavity of the
implant and the sulcus of the natural teeth maximum plate was (0.05mg/
ml) and (0.08mg/ml) respectively while the MBC for both bacterial groups
were (0.08mg/ml) and (0.1mg/ml) respectively. This study exposed that zinc
oxide nanoparticles were able to inhibit and kill the aerobic and anaerobic
bacteria.
Investigation of bioactivity, biocompatibility, and antibacterial properties of a hydroxyapatite- enhanced nanocomposite for dental applications
AuthorsXin Yan, 1,16, Ali ebrahimi2, Reza Mohammadi3, Mona Mohajeri Tehrani4, Pardis Chaboki5 , Mohammed Mustafa6, Almustafa Alhumadi7,8,9, I. B. Sapaev10,11,12, Ahmed Hjazi13 , Khadije Yousefi14 & Mojdeh Yousefi15
JournalScientific Reports
Date2025-08-05
AbstractThe prolonged setting duration of mineral trioxide aggregate (MTA) constitutes one of its principal
limitations. In contrast, Nano Fast Cement (NFC) emerges as an innovative nanocomposite
characterized by a rapid setting time. To develop an outstanding dental filler, hydroxyapatite
nanoparticles (NHA) were incorporated into NFC to evaluate their effects on setting time,
biocompatibility, bioactivity, and antibacterial properties. Specimens containing 0, 10, and 20
weight% (W%) hydroxyapatite were subjected to assessment utilizing the MTT assay for cytotoxicity,
the Gilmore needle for measuring setting time, scanning electron microscopy (SEM) and X-ray
diffraction (XRD) for microstructural and phase analyses, as well as antibacterial evaluations against
Enterococcus faecalis (PTCC 1394). Phase and microstructural investigations confirmed the formation
of hydroxyapatite. The addition of 10 and 20 W% hydroxyapatite significantly improved the specimens’
bioactivity while reducing toxicity by 20%. An increase in hydroxyapatite content corresponded with
a greater enhancement in bioactivity and a further reduction in toxicity. These findings indicate that
the addition of NHA did not detrimentally affect the physical properties of NFC; rather, it augmented
the filler’s bioactivity in vitro. Furthermore, the nanocomposite containing a higher concentration of
NHA exhibited superior inhibition of E. faecalis compared to NFC in minimum inhibitory concentrations
(MICs) and minimum bactericidal concentrations (MBCs) (p-value
<
0.001).
Keywords Biocompatibility
Nanocomposite, Nano fast cement, Dental filler, Nano hydroxyapatite, Bioactivity,
Gender differences in lower facial soft tissue thickness among different skeletal patterns, based on soft tissue cephalometric analysis
AuthorsAlmustafa Alhumadi, Thaer Jaber Al-Khafaji , Ali Mihsen Hussein Alyassiri , Wisam W Alhamadi
JournalWolters Kluwer - Medknow
Date2022-10-13
AbstractAbstract
Objective: Lower face soft tissue thickness and dentoskeletal features form the lower facial profile. Sagittal skeletal malocclusions with varying degrees of soft tissue thickness in males and females were herein examined using soft tissue cephalometric radiography.
Methods: Based on their dentoskeletal correlations, a total of 160 lateral cephalometric radiographs of adult males and females (n = 80) seeking orthodontic treatment were classified as class I (n = 40), class II division 1 (n = 40), class II division 2 (n = 40), or class III (n = 40). Holdaway analysis was used to assess soft tissue thickness in seven linear parameters.
Results: In class I, class II division 1, class II division 2, and class III dentoskeletal connections, males exhibited larger soft tissue thickness. They have an average lower lip thickness, chin depth H, and depth V for class I males. Males and females differed from one another when it came to the thicknesses of the upper and the lower lips. These lip thicknesses as well as the chin's width differed more between men and women in class II division 1. Except for upper lip strain, all measures in the class II division 2 sample males demonstrated a greater significance. In the class III sample, males also demonstrated more significance than females.
Conclusion: Males with various sagittal skeletal malocclusions demonstrated a significant difference in lower soft tissue thickness (characterized as thicker lower facial soft tissue) compared to female patients in class I, class II division 1, class II division 2, and class III malocclusions.
Keywords: Gender differences; sagittal skeletal malocclusions; soft tissue cephalometric analysis; soft tissue thickness.
An assessment of mineral concentration of dental enamel neighbouring hypothetical orthodontic brackets using X-ray microtomography
AuthorsThaer Jaber Al-Khafaji, Thaer Jaber Al-Khafaji
,
Bahn Agha
,
Almustafa Alhumadi
,
Wisam W. Alhamadi
,
David Mills
,
Graham Roy Davis
,
Alexander J. Cresswell-Boyes
,
Padhraig S. Fleming
JournalElsevier B.V.
Date2022-09-23
AbstractAbstract
Objectives
Differences in the mineral concentration (MC) level of dental enamel may represent a precursor of white spot lesions adjacent to fixed orthodontic brackets. The aim of the current in vitro study was to compare the MC level central, occlusal and cervical to orthodontic attachments.
Methods and Materials
A total of 16 enamel blocks were obtained from sound human premolar samples extracted for orthodontic reasons. The buccal portion of the dental enamel blocks was divided into central, occlusal, and cervical regions and then imaged and measured to calculate the level of MC using quantitative X-ray microtomography methods (XMT) at each site.
Results
There was a substantial variation in the mineral concentration with the lowest level being detected in the cervical region when compared with other regions. The MC of the gingival zone was significantly lower than that of the middle zone (P<0.05) and was insignificantly lower than that of the occlusal zone.
Conclusion
Within the limitations of the current study, it can be concluded that the cervical region of the permanent enamel had the lowest mineral concentration using XMT. The cervical region may therefore be more vulnerable to the development of white spot lesions (WSLs) adjacent to a fixed orthodontic appliance during orthodontic treatment.
Clinical significance
Using X-ray microtomography lower mineral concentration in the cervical region of the enamel was observed. This may make these areas particularly susceptible to demineralisation during fixed appliance-based orthodontic treatment and may influence the bond strength of fixed orthodontic attachments.
Management of a large venous malformation of the lower lip using dual-wavelength (810 nm and 980 nm) diode laser photocoagulation therapy: a case report
AuthorsHani M. Al-Kufi, Hani M. Al-Kufi, Abbas A. Y. Taher, Dhyaa M. J. Hirz Al-Deen & Almustafa Alhumadi
Journalspringer nature
Date2024-10-22
AbstractAbstract
Purpose
The aim of this study is to evaluate the effectiveness and safety of dual-wavelength (810 and 980 nm) diode laser photocoagulation therapy in the management of venous malformations.
Materials and methods
A 49-year-old female patient was referred to the Department of Laser at Naser Al-Mousawi Specialized Dental Center, complaining of a large, painless mass on her lower lip. The lesion was asymptomatic and covered a large portion of the lower lip. According to the patient, the lesion had been present since birth. Additionally, the size of the lesion had increased over the past 20 years. Upon clinical examination, the mass appeared dark purplish in color, soft, and compressible, indicating a low-flow vascular malformation. The patient was referred for a Color Doppler Ultrasound to evaluate the type and flow rate of the lesion. The results revealed characteristic features of a low-flow malformation (low flow and lack of pulsatility). The treatment was performed by photocoagulation using the QuickLase dual-wavelength (810–980 nm) diode laser. The laser parameters included a total power of 1.2 W, divided equally between the two wavelengths: 0.6 W from the 810 nm wavelength and 0.6 W from the 980 nm wavelength. The mode of operation was pulsed emission (100 ms pulse duration), and the diameter of the fiber optic tip was 400 μm. The fiber optic tip was not initiated and was positioned as close as possible to the lesion, without making contact. The treatment was done in two sessions, with the second session performed one month after the initial treatment to eliminate the entire lesion, followed by three-month and six-month follow-up periods.
Results
The results obtained from the study showed significant improvement. The initial swelling and discoloration resolved almost completely, and the tissue returned to a near-normal state with no residual effects or signs of recurrence observed during the three-month and six-month follow-up periods following laser photocoagulation therapy.
Conclusions
According to the study results, photocoagulation therapy using a diode laser is considered an effective method for managing vascular lesions due to its simplicity, high patient acceptance, minimal invasiveness, excellent healing, and fewer postoperative complications. Imaging, particularly color Doppler ultrasound, is crucial in diagnosing large vascular malformations, as it can identify flow patterns and determine the extent of the lesion. Regarding the number of treatment sessions, in cases of large vascular lesions, a single session may be insufficient to completely eliminate the lesion, often necessitating multiple sessions.