Effect of Urinary Bladder Sub mucosa in tendon reconstruction in horse
This study was planned to assess the efficacy of bovine urinary bladder matrix (UBM) in acceleration and enhancement of tendon healing, after experimentally induced-tendonitis, via tendon tearing of superficial digital flexor tendon (SDFT). This study was carried out on 12 adult healthy donkeys, which were divided randomly into two equal groups (six animals/group). Under the effect of tranquilizer and general anesthesia, a 3cmX0.3mm longitudinal intratendenous tearing and depth of 0.2mm, was induced in the middle third of SDFT at the metacarpal region of right forelimb, by a partial removal of tendon tissue and made of grove within the tendon core. In treatment group, a decellularized and disinfected scaffold as a strip of 3cmX0.3cm with depth of 0.2mm, which was prepared from the whole fresh bovine urinary bladders of slaughtered cows at the local abattoir as a urinary bladder extracellular matrix (UBM-ECM), was firmly embedded within the grove of the tendon. The same procedure of treatment group was repeated in the control group with exception of injection of 3ml sterile normal saline.