ضرغام حميد حيدر الحيدري

Peripheral nerve injuries (PNI) are frequently caused by several circumstances, including trauma and iatrogenic operations, and there are limited therapy options to help with functional recovery. This study aimed to compare in vivo adipose-derived stem cells (ASCs) to chitosan nanoparticles (CS NPs) in rabbit sciatic nerve defect regeneration. Twenty healthy rabbits of local breed randomly enrolled in two equal groups split into two groups at random: The critical size defect was bridged with a 14 mm conduit from acellular bovine urinary bladder impacted with chitosan nanoparticles (CS NPs) semi-gel 1mg/ml as the first group, and the critical size defect was bridged with mesenchymal stem cells (MSCs) 5x106 in the second group. At the end of 56 and 112 days, assessment of the nerve regeneration, motor and sensory function, and histopathological assessment of the sciatic nerve were investigated. According to the findings, the motor and sensory capabilities of the sciatic nerve increased faster in the mesenchymal stem cells (MSCs) group. In the mesenchymal stem cells group repairing a 10mm sciatic nerve defect, histopathological examination revealed basophilic nuclei of proliferated Schwann cells with thick myelin sheath, suitable orientation nerve fibers, dense axon population, large axon diameter, nerve fiber myelination, and low fibrous tissue at the epineurium compared to chitosan nanoparticles (CS NPs). It is concluded that effect mesenchymal stem cells (MSCs) promote regeneration of the sciatic nerve defect compared to chitosan nanoparticles (CS NPs).

The multipotent characteristic of rabbit adipose-derived stem cells makes them available and convenient sources for isolating mesenchymal stem cells. The aim of this study was to assess the differentiation in rabbit adipose-derived stem cells pre-committed to produce several mesenchymal lineages in response to inductive extracellular cues to multipotent stromal cells. Three grams of adipose tissue was taken from a subcutaneous region of the nape of the neck and was carefully isolated to obtain mesenchymal stem cells for expanded by fourth passage.

Twelve adult Awassi Rams about one year old were utility in this study. The induced SDF tendinitis in the LCSI of full thickness was created at the middle third of the palmar metacarpal forelimbs of the SDFT. The healing of tendinitis was evaluated depending on the clinical (lameness and local swelling), serological (FGF and VEGF), ultrasonographical and histopathological evaluation. The lameness values presented higher level of the significant differences (p ³ 0.05) in the control group more than the PRP group compere with the zero periods, but the level of PRP group rebounded to the normal degree in PRP group at 10th day with remained the lameness signs in the control group. The local swelling recorded the higher levels since 1st weeks in both groups, and rebounded to the normal levels at 7th week in control group, while at 8th week in PRP group. The FGF values didn’t record any significant difference of all the periods in the control group except the last three periods after the 12 weeks, also the VEGF levels were similar to the FGF levels data. The echogenicity of control group was enhanced gradually at 1st, 2nd and 4th months without completely repair, but the enhancement of PRP group was typical as a regenerative healing. The histopathological evaluation was matching to the ultrasonographical evaluation but the image was under the light microscope. The conclusion of assay is the competence of PRP to produce the regenerative healing in acute tendinitis of sheep.

Aim: The present study was designed to demonstrate the autoligation (AL) of vas deferens and the excision-ligation (EL) technique to generate vasectomized rams to reduce the complications, operative time, and price of the vasectomy techniques. Materials and Methods: A total of 12 healthy and mature Iraqi Awassi rams were used, which divided into two groups, six rams for each one. The former group was performed the EL technique while the latter group, the AL of vas deferens technique was used. Results: The results of the present study found that both techniques were same with the reproductive efficient examinations that mean the two techniques had same ability to close the male genital passage for teaser rams preparation. However, the methods were different with the histopathological changes, operation time, prices, and complications, which were minor in the AL of vas deferens compared with the EL technique. Conclusion: The AL technique of vas deferens to prepare teaser animal is recommended over the EL technique due to different aspects such as cost, fewer complications, and active teaser for a long period are the main aspects of AL technique. Keywords: ram infertility, testes, vas occlusion, vasectomy techniques.

Propolis “bee glue” is a nature substance, it is collected from combing of bee hive frames. It has several medical remedies such as anti-microbial, anti-spasm, local anesthetic, and wound healing. The cutaneous wounds repair still remain a big challenge because of skin ability to heal as scar. Therefore, this study aimed to evaluate the Iraqi Propolis to regenerate wound healing. To estimate the total leukocyte count (TLC), wound diameter, histopathological examination of the skin and evaluation of cutaneous fibroblast growth factor (FGF2 ) level. Results revealed that the TLC recorded significant elevating in control group for all periods after induced wound. While the treated group was returned to normal level in (21 and 28 days). The wound diameter was reduced in the treated group compered with the control group. The histopathological changes of treated group rebounded to a semi-natural pattern in analogical periods compared with control group. The FGF2 level suffered from rapid elevating with small period in propolis group more than control group to heal the wound. Also its increase rebounded the healed cutaneous wounds at last periods of the treated group to regeneration. This assay demonstrated propolis is not only enhancement and acceleration of the wound healing in dogs but also prevent contamination, relive pain, avoid scar formation and minimize hemorrhage therefor it is a typical drug for a topical administration of the wound treatment.

Objectives: To describe the efficacy of the bone marrow stromal cells (BMSCs) implantation on peripheral nerve regeneration in a dog model. Methods: Ten local breed dogs were divided into two equal group (n=5). In the epineural repair group (control group), the left sciatic nerve was skeletonized from the sciatic notch to the point of bifurcation, with the nerve been transected at the mid-shaft of the femoral bone and repaired with six epineural nerve sutures (ENS group). In the treated group, the epineural repaired nerve was implanted with BMSCs in the proximal and distal segments of the transected sciatic nerve (BMSCs group). Assessment of the nerve regeneration was based on functional recovery (motor and sensory). Results: showed that the motor and sensory functions acceleration of sciatic nerve improved more rap idly in the BMSC group. The histopathological examination revealed basophilic nuclei of proliferated Schwann cells with thick myelin sheath, good orientation nerve fibers, dense axon population, large diameter of axon and myelination of nerve fibers and low fibrous tissue at the epineurium improved in the BMSCs group. Conclusion: the results of the examination showed that the treated group had the best regeneration and functional recovery.

تقييم طلاب السنة الخامسة في الحالات السريرية

أقام فرع السريريات تقييم امتحان الجراحة العملي للكورس الثاني للمرحلة الخامسة و تم الامتحان العملي في صالة العمليات الجراحية في كلية الطب البيطري/جامعة الكوفة وبإشراف المدرس المساعد ضرغام الحيدري حيث تم تقييم الطلبة بصورة علمية و عملية لكفاءتهم في إجراء العمليات الجراحية و من خلال توزيع الطلبة على مجاميع وتم تخصيص عملية جراحية لكل مجموعة حيث كان التقييم في استخدام الأدوات الجراحية وكذلك ملاحظة نتائج العمليات.

اقيم في قسم الجراحة والتوليد فرع السريريات عملية فتح كرش Rumenotomy لاحد العجول في الحقول الحيوانية وتمت العملية باشراف الدكتور حسين كريم والدكتور ضرغام الحيدري مع طلبة المرحلة الخامسة

في مجال التعاون المشترك بين الجامعات العراقية نظمت كليتنا زيارة علمية لطلبة المرحلة المنتهية إلى جامعة دهوك -كلية الطب البيطري في صباح يوم الخميس الموافق ١٨ / ٤ . حيث أَستقبل الوفد من قبل عماده الكلية متمثله بالسيد عميد الكلية أ.م.د.لقمان طيب عمر ومعاونيه و تطرق السيد العميد في بداية اللقاء حول تاريخ تاسيس الكلية والخطط السنوية والمستقبلية وتباحث الوفد سبل التعاون المشترك بين الكليتين وتم في الزيارة التعرف على الإمكانيات العلمية والبحثية و المختبرية المتوفرة في جامعة دهوك