Benner
زهره محسن الجميلي ( أستاذ مساعد )
كلية العلوم - علوم الحياة
[email protected]
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Allele C of the IL-12p40 1188A/C Single Nucleotide Polymorphism Associates with the Increased Susceptibility to Tuberculosis
تحميل
بحث النوع:
طب التخصص العام:
ZahraMuhsin Ali Department of Biology, Faculty of Science, Kufa University, Iraq اسم الناشر:
Bushra Jabbar Altamimi Departmant of Anatomy, College of Medicine, Babylon University, Iraq اسماء المساعدين:
American Journal of Research Communication www.usa-journals.com الجهة الناشرة:
American Journal of Research Communication, 2015, 3(11): 161-169} www.usajournals.com, ISSN: 2325-4076.  
2015 سنة النشر:

الخلاصة

Abstract Background: Tuberculosis (TB) represents a major public health problem worldwide. Wide variation occurs in the incidence of TB in different races and ethnicities suggesting a role for genetic factors. Aims: This study aimed to find out the relationship between single nucleotide polymorphism (SNP) in interleukin-12 (IL-12p40 1188A/C) with TB and serum levels of IL-12 in Iraqi patients. Subjects and Methods: This case/control study involved 55 TB patients and30 age-matched unrelated healthy controls. Blood samples were obtained from the study population. DNA was extracted from leukocytes, and IL-12p40 1188A/C gene was amplified with sequence specific primer polymerase chain reaction (SSP-PCR) using a specific set of primers. Serum levels of IL-12 was estimated using enzyme-linked immunosorbent (ELISA) technique. Results: The IL-12p40 1188A/C SNP appeared in three genotypes: AA, AC and CC. These genotypes represented 236.37%, 40% and 23.64% respectively among patients and 56.37%, 36.7% and 6.6% among control, with significant difference for the heterozygous genotype (OR= 5.525, 95%CI=1.090-28.003, P=0.039), and insignificant difference for the homozygous mutant genotype (OR=3.250, 95%CI=0.621-17.013, P=0.163). Carriers of CC genotype had higher level of IL-12p40 (219.45± 91.567 pg/ml) than either AC genotype carriers (166.705± 76.211 pg/ml) or AA genotype carriers (107.321± 68.358 pg/ml) with significant differences among the three groups (P<0.05) Conclusion: The C allele of IL-12p40 1188A/C SNP can be considered as a risk factor for TB in Iraqi patients. Moreover, this allele causes increase production in IL-12p40. Keywords: tuberculosis, single nucleotide polymorphism, IL-12p40 1188A/C {Citation: Bushra Jabbar Altamimi, ZahraMuhsin Ali. Allele C of the IL-12p40 1188A/C single nucleotide polymorphism associates with the increased susceptibility to tuberculosis. American Journal of Research Communication, 2015, 3(11): 161-169} www.usajournals.com, ISSN: 2325-4076.