Benner
تغريد عبد الكريم المخزومي ( مدرس مساعد )
كلية العلوم - التحليلات المرضية
[email protected]
07816051122
 
 
 
PCR DETECTION OF CTX-M GENES CODING ANTIBIOTIC RESISTANCE IN Klebsiella spp. ISOLATES IN AL-NAJAF PROVINCE, IRAQ
تحميل
بحث النوع:
علوم التخصص العام:
AL-MUHANNA A.S.1 اسم الناشر:
AL-MAKHZOOMY T.A.2 اسماء المساعدين:
International Journal of Microbiology Research الجهة الناشرة:
ISSN: 0975-5276 EISSN:0975-9174  
2013 سنة النشر:

الخلاصة

One hundred and fifty five of Gram-negative-lactose fermented bacteria grown on MacConkey agar were collected from two main hospitals in Al- Najaf province. The isolates were identified according to cultural characteristics, biochemical activities and Vitick -2 system. 43(27.7%) isolates were identified as Klebsiella spp. Isolates, represented by 35(81.3%) isolates K. pneumoniae subsp. aerogenes, 4(9.3%) isolates Klebsiella pneumoniae subsp. pneumoniae, 2 (4.6%) isolates Klebsiella oxytoca, as well as one isolate for each of K. pneumoniae subsp. ozaenae and K. pneumoniae subsp. rhinoscleromatis. The susceptibility of Klebsiellae isolates to 20 antibiotics were tested, using disc diffusion method; the results have revealed that Klebsiellae isolates were highly resistant to most common antibiotics. The ability of Klebsiellae isolates to produces extended β-lactamase were tested, the results have revealed that 26 (60.4%) isolates produce ESBLs. The ability of Klebsiellae isolates to have CTX-M gene was tested; PCR amplification results have shown that 15 of Klebsiellae isolates were possess CTX-M lactamase gene.