الخلاصة

new technological tools are developed rapidly, mainly HTML5 and JavaScript, making users familiar with designing more accurate and accepted primer for PCR. The complicated primer designing options can be excluded or modified by a number of mouse clicks process.[...] the researcher recommends PCR Designer for Restriction Analysis of SNPs, a web server for imagination PCR primer design on well-known reference SNP ID number (" rs#";" refSNP cluster").[...] the mixing of new technologies, the commonly used primer design software, primer 3 plus and the blast-checking program will be a helpful image for genotype SNPs detection PCR primer design tool.