الخلاصة

2-[4-Chloro-2-methoxy phenyl azo]-4,5-diphenyl imidazole used as a new complexing organic reagent for extraction and determination of copper (II) in different samples include plants leaves, soil and water from different position in AlNajaf governorate as well samples of human serum, cow liver and kidney, vitamins and spices. All these measurement carried out at the optimum condition for complexation reaction. Beer's law is obeyed over the concentration rang 0.5-30µg/mL with a detection limit 8×10-5 µg.mL-1 and molar absorptivity 8459.79L.mole-1.cm-1. Sandell’s sensitivity 7.5×10-8µg.cm-2.