صالح مهدي الحفاجي ( أستاذ )
كلية الطب - طب عام
[email protected]
The significant ofC677Tmethylenetetrahydrofolate reductase MTHFR gene mutation in Iraqi patients with type 2 diabetic nephropathy
بحث النوع:
طب التخصص العام:
صالح مهدي الخفاجي اسم الناشر:
انوار مدلول الجنابي و شهاب احمد فارس اسماء المساعدين:
International journal of engineering and scientific research الجهة الناشرة:
مجله اجنبيه  
2017 سنة النشر:


Abstract This study aimed to address the association of C677T point mutation of MTHFR gene with Diabetic Nephropathy (DN) in Iraqi type 2 diabetes mellitus patients (DM). We examined age; gender; duration of diabetic, Body mass index, glycosylated hemoglobin (HbAIc), albuminuria, blood sugar (RBS), homocysteine, serum urea and serum creatinine and MTHFR (C677T) gene polymorphism in 65 (DN) and 70 (DM) patients. After DNA extraction from blood samples of all participants the PCR-RFLP technique were applied for detection of MTHFR gene polymorphism.We found the means of serum homocysteine concentration, proteinuria, blood urea, serum creatinine which were significantly increased in DN patients when compared with DM patients (P˂0.05). The miner allele frequency of T genotype was significantly increased in DN patients compared to the DM patients (P˂0.0001) and the risk of DN was higher by 8.09 folds in homozygous allele genotype (TT) when compared with wild genotype (CC) ( OR 8.09, 95% CI= 2.6-24.8 P= 0.0003).We concluded that the TT genotype of MTHFR gene is increased the risk of DN in type2 diabetic nephropathy patients of Iraqi population.