Benner
سحر عبد الرضا الحار ( أستاذ مساعد )
كلية الطب - طب عام
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Leukotriene Synthesis Inhibitors Modulate Atherosclerosis Progression In Hypercholestrolemic Rabbits.
بحث النوع:
طب التخصص العام:
Najah R Hadi اسم الناشر:
Bassim I Mohammad2, Ihsan M Ajeena1, Ahmed Mahmood1, Sahar A Majeed1 and Tahir Hussain اسماء المساعدين:
Research Journal of Pharmaceutical, Biological and Chemical Sciences الجهة الناشرة:
July– August 2015 RJPBCS 6(4) Page No. 362  
2015 سنة النشر:

الخلاصة

This study was undertaken to evaluate the effect of zileuton on the progression of atherosclerosis. A total of 28 local domestic rabbits were used in this study and were randomized into to four groups. Group I (normal control) received standard chow diet, other three groups received atherogenic diet (2% cholesterol) and treated as follows: Group II (atherogenic control) received no treatment, Group III (vehicle control) received ethanol 10 % as solvent, Group IV received zileuton 150 mg\kg daily. Blood samples were collected at the end of experiment (8 weeks) for measurement of total cholesterol (TC), serum triglycerides (TG), HDL-C, plasma high sensitive C-reactive protein (hsCRP), plasma malondialdehyde (MDA) and plasma reduced glutathione (GSH). Immunohistochemical analysis (VCAM-1, MCP-1, IL17 and TNF-α,) and histopathologic assessment of aortic atherosclerotic changes were also performed. Compared to NC, levels of lipid profile, atherogenic index, hsCRP, and MDA are increased while GSH were decreased in animals on atherogenic diet (p< 0.05). Immunohistochemical analysis showed that aortic expression of VCAM-1, MCP-1, IL17 and TNF-α were significantly increased in AC group compared to NC group (p<0.001). Histopathologic finding showed that animals on atherogenic diet have significant atherosclerotic lesion compared to NC group. Compared to AC group zileuton don’t have significant effect on lipid profile. Zileuton causes statistically significant reduction in hsCRP and MDA (p<0.05). Zileuton treatment causes significantly increase the level of GSH. Zileuton treatment significantly reduced aortic expression of VCAM-1, MCP-1, IL17 and TNF-α (p<0.005). Histopathologic examination of aortic arch showed that zileuton significantly reduced atherosclerotic lesion (p<0.005). It thus can be concluded that zileuton reduces lipid peroxidation, systemic inflammation and aortic expression of inflammatory markers used in this study and hence reduce the progression of atherosclerosis. Keywords: atherosclerosis, zileuton, oxidative stress.