محمد محسن الرفيعي ( أستاذ مساعد )
كلية العلوم - علوم الحياة
[email protected]
Rapid Identification of Dermatophytes Isolated from Clinical Specimens from Dermatophytosis Patients by Application of the PCR-RFLP method.
بحث النوع:
علوم التخصص العام:
Adnan Hamad Al-Hmadani1 اسم الناشر:
Muhsin Abdulhusien Al-Dhalimi2*, and Muhammad Muhsien Abdulhusien Alrufae3 اسماء المساعدين:
Research Journal of Pharmaceutical, Biological and Chemical Sciences الجهة الناشرة:
RES J PHARM BIOL CHEM SCI Volume 7, Issue 4, 2016 (July - August)  
2016 سنة النشر:


ABSTRACT Dermatophytes are a group of closely related fungi that invade keratinized tissue (skin, hair and nails). In the present study, a molecular technique was designed to optimize the polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) method targeting internal transcribed spacer (ITS) regions in 18S ribosomal DNA (rDNA) of fungi for rapid detection and identification of dermatophytes. A total of 216 specimens from skin, hair and/or nail were collected from 150 patients with dermatophytosis. Specimens were inoculated on Sabouraudʹs Dextrose Agar (SDA). A small portion of each fungal colony was further studied by RFLP analysis of the PCR-amplified ITS region of rDNA. PCR amplicons and were visualized by electrophoresis on a 2% agarose gel after digestion by restriction enzymes including HinfI and HaeIII. Nine dermatophyte species including M. canis, T. mentagrophytes, T. tonsurans, E. floccosum, T. rubrum, T. ajelloi, M. gypseum, M. audouinii and T. schoenleinii were identified based on the colony morphology and microscopic criteria. Specific PCR products and RFLP patterns allowed for the rapid identification and reliable differentiation at the genus or species level. Our results showed that this technique is a rapid and reliable tool which allows identification of major pathogenic dermatophytes isolated at the species level. Keywords: dermatophytes, PCR-RFLP, skin.