الخلاصة

ABSTRACT Many efforts had been focused on the therapeutic approach of cancer for a long time by studying cytotoxic nanoparticles' activities. In this study, a mix of PVA 60% and PVP 35 %, with 5% reinforced bioceramic triple (ZnO, MgO, Tio2) was applied against LS 174T colorectal adenocarcinoma cell line. This study was planned to evaluate MZT cytotoxicity by thiazolyl blue tetrazolium bromide (MTT) cytotoxicity test on LS 174T cell line in compared with chemotherapeutic drugs that were included Doxorubicin (DOX) and 5-flourouracil (5FU). These nanoparticles were purchased from Skyspring Nanomaterials, Inc. USA, with 10-30 nm in diameter and 99.8%.purity. The size was further confirmed with X-ray Diffraction (XRD), Energy Dispersive X-ray Spectroscopy (EDS) and Scanning Electron Microscopy (SEM). The results showed that MZT nanocomposite was effectively and significantly inhibited the cell proliferation (p<0.0005) by decreasing the viability of LS 174T cells at different concentrations involved 1, 10, 100, 1000 and 2000 μg/ml, with 5.698 μg/ml half maximal inhibitory concentration (IC50) of. DOX and 5FU IC50 values were 27.409 μg/ml and 62.84 μg/ml, respectively. It was concluded that MZT nanocomposite have an anticancer effect against LS 174T colorectal carcinoma and this type of cell line was good enough for applying MTT cytotoxicity assay. Keywords:LS 174T colorectal adenocarcinoma, Cell culture, MTT Assay, Nanoparticles.