الخلاصة

Abstract Many efforts have focused on the therapeutic approach of cancer for a long time by studying cytotoxic activities of plant constituents. This study was planned to evaluate cytotoxicity of Nitrochalcone derivative (NCD) on rhabdomyosarcoma cell line (RMS) as compared with methotrexate and Doxorubicin, then assessment of monoterpene combination with NCD. The results of current study were showed that NCD was effectively inhibited the cell proliferation by decreasing the viability of RMS cells at different concentrations involved 0.5, 2.5, 10, 20, 30, 40, 50, 60 and 70 μg/ml with IC50 of 2.117μg/ml, while Doxorubicin (DOX) and Methotrexate (MTX) IC50 values were 0.553μg/ml and 0.8708μg/ml respectively. This study was also revealed combined effect of NCD with monoterpene (IC50 = 2.0667). We concluded that NCD have an anticancer effect that can be modulated by monoterpenes. Keywords: Nitrochalcone derivative; Cytotoxicity assay; RMS cell line