الخلاصة

ABSTRACT This study was planned to evaluate the prevalence and immunological aspects of Herpse simplex virus2 (HSV2) in the semen of men with genitourinary tract infection compared with control group in Al-Najaf City /Iraq. The attempts to investigate the sexually transmitted diseases (STDs) among individuals inhabit in highly restricted religions society could consider a difficult mission, therefore, this paper could provided a novel information about the status of that subject. Primarily, 115 semen samples were collected from cases of genitourinary tract infections, their ages ranged from 20-65 years old during the period from May to October 2015.Fifty samples were also obtained from a comparable 50 healthy persons, all investigated by quantitative real-time polymerase chain reaction (qPCR). RT-PCR directed to certify HSV2 infections. Age groups it was comprised 20:40(50%) with significant statistical differences (p<0.05). The investigation of certain immunological parameters as Interleukine-2 (IL-2), Tumour necrosis factor (TNF-α) and secretory Immunoglobulin (sIgA) revealed elevation during the HSV2 infection but without significant statistical differences (p> 0.05). We concluded that HSV2 is positively correlated and the patient age may play an important role in increasing the frequency of genitourinary tract infection. RT(PCR)is complementary to cell culture technique in diagnosis of HSV, and it`s preferred to use specific primers of viral virulence factors and different cell lines for monitoring viral pathogenicity and therapeutic approaches is recommend to study . Keywords: Herpes simlexvirus2, immunological parameters, real time PCR, Semen