Benner
د.حسين حميد عبد الله ( مدرس )
كلية العلوم - علوم الحياة
[email protected]
 
 
 
Characterisation and optimization of AgNPsbiosynthesis by Klebsiella Pneumoniae HHHS1 isolated from silver rich environmenthttp
تحميل
بحث النوع:
علوم التخصص العام:
Dr. Hussein Hameed Hassan اسم الناشر:
Dr. NawfalHussein Aldujaili اسماء المساعدين:
BIOCHEMICHAL AND CELLULAR ARCHIVES الجهة الناشرة:
BIOCHEMICHAL AND CELLULAR ARCHIVES ISSN:_0972-5075  
2018 سنة النشر:

الخلاصة

Silver rich soil samples were screened for the potent bacteria for AgNPs biosynthesis. Silver resistant bacteria are the potent for AgNPs biosynthesis. K. Pneumoniae strain HHHS1 was isolated and characterized using 16sRNA gene sequencing and VITEK-II, and used for extracellular synthesis of AgNPs by cell free supernatant of BHI culture media. Conditions of synthesis were optimized and biogenic AgNPs were characterized physically using SEM, EDS, AFM, and XRD, and biologically by examining for antibacterial, antibiofilm, and antioxidant activity. Sequencing of 16sRNA gene and distance tree analysis revealed that the isolated K.pneumoniae is a new strain submitted to the NCBI as HHHS1. SEM, EDS, AFM, and XRD analysis for biogenic AgNPs fabricated by this strain revealed spherical, monodispersed NPs with average size 21.9 nm. Optimum conditions of the synthesis environment were found: pH 8, 370C, 8h, substrate concentration was found proportional to AgNPs characteristic.