Benner
أ. م. انعام جواد العباسي ( أستاذ مساعد )
كلية التربية للبنات - علوم الحياة
[email protected]
07812185409
 
 
 
genotype and phenotype detection of E. coli isolated from children suffering from urinary tract infection
تحميل
بحث النوع:
تربية التخصص العام:
Anaam Jawad Alabbasy اسم الناشر:
اسماء المساعدين:
scopus الجهة الناشرة:
IQ 4  
2018 سنة النشر:

الخلاصة

One hundred clinical urine samples were obtained from patients suffering from UTI during the period from 1, August, 2017 to 9, October, 2017. Methodology: The identification of the E. coli isolates was depended on colonial morphology, microscopic examination and biochemical tests as a primary identification. The final identification was performed by PCR technique with Mdh. In addition antibiotic genes were used such as (bla-CTM, blatem-1, aac (6)-Ib-crandsul-1). Result: Rendering to the results obtained according to the morphological ,cultural and biochemical characters from 100 sample only 80 (80%) isolate was identify as E. coli. According to the results obtained by PCR, Mdh gene that encode was found in 56 (70%) isolates in E. coli. The results of PCR revealed that the percentage of bla-CTX-M gene among tested isolates reached to 30/56(53.57%), 10/56(17.85%) for blaTEM-1, 13/56(23.2%) for aac (6')-Ib-cr and 33/42 (78.57%) for Sul-1gene. Conclusion: The study investigated the antibiotic resistance genes of E. coli which play a major role in pathogen city .