Benner
إحسان فلاح بيرم ( مدرس )
كلية الصيدلة - صيدلة عام
[email protected]
 
 
 
Sulfation of Catecholamines and Serotonin by SULT1A3 Allozymes
تحميل
بحث النوع:
صيدلة التخصص العام:
Ahsan F. Bairam اسم الناشر:
Mohammed I. Rasool, Fatemah A. Alherz, Maryam S Abunnaja, Amal A. El Daibani, Saud A. Gohal, Katsuhisa Kurogi, Yoichi Sakakibara, Masahito Suiko, Ming-Cheh Liu اسماء المساعدين:
Biochemical Biochem Pharmacol الجهة الناشرة:
Biochem Pharmacol. 2018 May;151:104-113. doi: 10.1016/j.bcp.2018.03.005. Epub 2018 Mar 8.  
2018 سنة النشر:

الخلاصة

Abstract Previous studies have demonstrated the involvement of sulfoconjugation in the metabolism of catecholamines and serotonin. The current study aimed to clarify the effects of single nucleotide polymorphisms (SNPs) of human SULT1A3 and SULT1A4 genes on the enzymatic characteristics of the sulfation of dopamine, epinephrine, norepinephrine and serotonin by SULT1A3 allozymes. Following a comprehensive search of different SULT1A3 and SULT1A4 genotypes, twelve non-synonymous (missense) coding SNPs (cSNPs) of SULT1A3/SULT1A4 were identified. cDNAs encoding the corresponding SULT1A3 allozymes, packaged in pGEX-2T vector were generated by site-directed mutagenesis. SULT1A3 allozymes were expressed, and purified. Purified SULT1A3 allozymes exhibited differential sulfating activity toward catecholamines and serotonin. Kinetic analyses demonstrated differences in both substrate affinity and catalytic efficiency of the SULT1A3 allozymes. Collectively, these findings provide useful information relevant to the differential metabolism of dopamine, epinephrine, norepinephrine and serotonin through sulfoconjugation in individuals having different SULT1A3/SULT1A4 genotypes.