عدنان كريم السلامي ( أستاذ مساعد )
[email protected]
Dissemination of TEM and SHV Extended-Spectrum Beta-Lactamases in clinical isolates of Escherichia coli.
بحث النوع:
صيدلة التخصص العام:
Adnan Kareem Abd اسم الناشر:
اسماء المساعدين:
Al-Kufa University Journal for Biology الجهة الناشرة:
ISSN: 20738854 23116544 Publisher: University of Kufa Faculty: Science Language: English This journal is Open Access  
2012 volume:4 issue: سنة النشر:


Abstract P_To examine the dissemination of blaTEM and blaSHV genes in Escherichia coli isolate from patients with significant bacteriuria. Methods_ During the study period, antibiotic susceptibility patterns were studied in a total of 38 isolates of E. coli. The isolates also examined to determining the presence of blaTEM and blaSHV genes by PCR assay. Conjugation experiments were performed by using rifampicin resistance E. coli MM294 as the recipient. Results_ All isolates were resistance to at least five of the antibiotics tested (multidrug resistance, MDR) and sensitive to imipenem. PCR assay revealed that 57.1% and 14.3% of the isolates carried blaTEM and blaSHV genes, respectively, additionally 9.5% of the isolates harbored both genes. Conjugation experiments and PCR trials confirmed that the blaTEM positive E. coli isolates tested were able to transfer thisurposgene in transconjugants. Conclusions: There was a high occurrence of MDR isolates in patients with significant bacteriuria and theblaTEM gene was common among isolates.